PolyLC Inc.

PolyLC specialize in solving the most difficult application problems:

Introducing ERLIC!

Gradient chromatography without the gradient! Separate peptides, nucleotides and amino acids socratically!

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ERLIC of Phosphopeptides

Phosphopeptides can also be isolated from tryptic digests and separated with high resolution.

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The Poster form HPLC 2008

Featuring volatile solvents and SPE-ERLIC for phosphopeptides.

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Mixed-bed Ion-Exchange Columns for Intact Proteins

• Proteomics: PolyLC PolySULFOETHYL A™ is the standard material for the SCX-RPC sequence for multidimensional LC of complex tryptic digests.
• QC: Analysis of closely-related protein variants.
• Metabolomics: Analysis of polar solutes in cell and plant extracts; quick isocratic analysis of amino acids; etc.
• Hemoglobin analysis: PolyLC PolyCAT A™ columns are the gold standard.
• Prion protein assay in blood samples.
• Membrane protein isolation.
• Separation by size of solutes as small as amino acids.
• PCR reactions: HPLC alternative to gels.

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Literature

• ASMS 2008 Poster
• HPLC 2008 Poster 05-2008
• ERLIC of Phosphopeptides Bulletin 02-2008
• Mixed-Bed Ion Exchange of Intact Proteins Bulletin 02-2008
• ERLIC Bulletin 6-2007
• ASMS 2007 ERLIC POSTER
• Bibliography 4-5-2006
• ABRF 2003 SEC Poster
• ABRF 2004 Poster
• ISPPP 1996 CEX Poster
• Metabolomics Brochure 2004
• Nucleic Acids Bulletin Revision 3, 6-3-03
• Proteomics 2006 Bulletin
• Proteomics Bulletin 2-2004 MG
• Proteomics Update 2005
• SEC Book Chapter Version 1

Applications

• Electrostatic Repulsion - Hydrophilic Interaction Chromatography (ERLIC)
• Hemoglobin Analysis
• Protein Variant Analysis
• Ion Exchange of Proteins with Organic Solvents
• Oligonucleotides and PCR Products
• Metabolomics, Amino Acids, and Polar Small Solutes
• Proteomics
• Hydrophilic Interaction Chromatography (HILIC)
• Size Exclusion Chromatography (SEC)
• Assay of Pathogenic Prion Proteins
• Isolation of Proteins and Peptides from Extracts and Natural Products
• Solid Phase Extraction